Course Content
Introduction to Mushroom Cultivation
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Introduction
Classification
Stages of mushroom
Classification of mushroom
Importance of Mushroom
Test to identify poisonous mushroom
How to identify poisonous Mushroom ?
Some of the well known poisonous mushrooms
Mushroom poisoning.
Cultivated Edible Mushroom
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1. Pleurotus spp.: “Kanye Chayau” or Oyster Mushroom.
Morphological characters
2. Agaricus spp. : “Gobre chayau” in Nepali and white button mushroom in English.
Morphological structure/characteristics
3. Volvariella spp/Paddy straw mushroom.(Parale chyau).
Morphological characteristic
4. Lentinula spp. (Shiitake)
Wild mushroom
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Introduction
Ganoderma Spp.(Red mushroom).
Morchella spp.( Gucchi chayau.)
A. Morchella esculenta : “Seto Gucchi” or White Morels
B. Morchella conica : “Kalo Gucchi” or Black Morels
C. Yarshagumna (Cordyceps sinensis): Caterpillar Fungus
D. Armillaria mellea: Honey Fungus
Cultivation of Agaricus spp.( Button mushroom)
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1. Spawn:
2. Composting:
3. Spawning.
4. Spawn running.
5. Casing
Time of casing:
Care after Casing:
6. Harvesting / Picking:
7. Grading:
8. Processing of Mushrooms:
Cultivation practice of Pleurotus spp
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Introduction
MATERIALS AND METHODS
1. Selection of Substrate
2. Sterilization
3. Inoculation
4. Incubation:
5. Observation:
6. Harvesting:
Cultivation of Volvariella Paddy Straw Mushroom (“Parale Chyau”)
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Introduction
Nutritional requirement
Environmental Requirement:
Substrate:
Cultivation Method:
1. Natural cultivation:
Harvesting and Marketing:
2. Room/Artificial cultivation:
Conditions for increasing or decreasing the yield of the mushroom:
Cultivation practice of Shitake Mushroom.
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Substrate used for spawn
Materials required
Drilling in wood and spawning procedure
Management of bed log
Spawn (Mushroom seed)
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Introduction
Essential qualities of good spawn:
Types of spawn:
Other Types of Mushroom Spawn
Isolation and maintenance of Pure Culture of Mushroom
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Isolation techniques for getting pure cultures and their maintenance:
1. Spore Culture Technique:
2. Tissue Culture Technique:
Sub-culturing:
Grain Spawn Preparation:
Diseases of Mushroom
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Green mold:
II. Dry Bubble/Verticillium spot:
III. Wet Bubble:
IV. Cobweb mold or Dactylium Mildew
Insect and pests of Mushroom
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a. Sciarid flies:
b. Phorids:
c. Mites:
d. Nematodes:
Learn Mushroom Cultivation with Braimy – B.Sc Agriculture
About Lesson

– A small bit from the pileal region is cut with the help of a sterilized blade or scalpel,

– Washed several times in sterilized distilled water

– Dried in a clean tissue paper before inoculating aseptically on a Petri plate or tube containing suitable culture medium.

– The inoculated Petri plates are incubated at 25 ± 1 °C for 6-12 days and observed at different intervals for the mycelial growth.

– All Petri plates / glass tubes showing contamination should be discarded and only the ones with pure growth should be retained for further use.

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