For fresh materials

• Grind the plant material either with using a small volume of suitable buffer or organic solvent or a liquid nitrogen.
• Filter and obtain a plant extract.
• Homogenize the extract with menthol and allow to stand for 24 hours at ordinary temperature.
• Filter and get obtain a filtrate.

For dried materials

• Powder the plant materials.
• Moisten sufficiently with water
• Filter and obtain filtrate.
• Add HCl or H2SO4
• Mix the filtrate with lime solution (NaOH or Na2CO3).
• Precipitation
• The solid mass obtained and it carried out with either methanol or benzene or petroleum ether
• Shaken or allowed to isolate.
• Alkaloid isolated by crystalline chromatography or ion exchange techniques.